Title:Zamzam Water Mitigates Cardiac Toxicity Risk through Modulation of GUT
Microbiota and the Renin-angiotensin System
Volume: 30
Issue: 14
Author(s): Ryan Adnan Sheikh, Mohammad Shahid Nadem, Turky Omar Asar, Mohammed A. Almujtaba, Salma Naqvi, Fahad A. Al-Abbasi, Naif Abdullah R. Almalki, Vikas Kumar and Firoz Anwar*
Affiliation:
- Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Keywords:
Doxorubicin, GUT microbiota, dysbiosis, zamzam, zinc, CRP, angiotensin, renin.
Abstract:
Background: Cardiovascular diseases (CVDs) continue to exert a substantial global influence in
specific areas due to population growth, aging, microbiota, and genetic/environmental factors. Drinking water
has a strong impact on the health of an individual. Further, emerging evidence has highlighted the therapeutic
potential and benefits of Zamzam water (Zam).
Objective: We investigated the influence of Zam on doxorubicin-induced cardiac toxicity, elucidating its consequential
effects on GUT microbiota dysbiosis and hepatic and renal functions.
Methods: Male rats were categorized into four groups: Group 1 as Normal control (NC), Group 2 as Zamzam
control (ZC), Group 3 Disease control (DC) and Group 4 as Therapeutic control (DZ) treated with Zam
against doxorubicin-induced disease at a dose of 1mg/kg boy weight) intraperitoneally (i.p).
Results: Significant dysbiosis in the composition of GM was observed in the DC group along with a significant
decrease (p < 0.05) in serum levels of Zinc, interleukin-10 (IL-10), IL-6 and Angiotensin II (Ang II),
while C-reactive protein (CRP), fibrinogen, and CKMB increased significantly (restoration of Zinc ions (0.72
± 0.07 mcg/mL) compared to NC. Treatment with Zamzam exhibited a marked abundance of 18-times to 72%
in Romboutsia, a genus of firmicutes, along with lowering of Proteobacteria in DZ followed by significant
restoration of Zinc ions (0.72 ± 0.07 mcg/mL), significant (p ˂ 0.05) reduction in CRP (7.22 ± 0.39 mg/dL),
CKMB (118.8 ± 1.02 U/L) and Fibrinogen (3.18 ± 0.16 mg/dL), significant (p < 0.05) increase in IL-10 (7.22
± 0.84 pg/mL) and IL-6 (7.18 ± 0.40 pg/ml), restoration of Ang II (18.62 ± 0.50 nmol/mL/min), marked increase
in renin with normal myocyte architecture and tissue orientation of kidney, and restoration of histological
architecture of hepatocyte.
Conclusion: Zam treatment mitigated cardiac toxicity risk through the modulation of GUT microbiota and the
renin-angiotensin system and tissue histology effectively.
