Title:Qualitative Analysis and Anti-oxidant Potential of Ethanolic Extract of
Manilkara zapota (L.) P. Royen Leaves
Volume: 10
Author(s): Priyanka Sharma, Aakash Deep*, Harish Kumar, Devendar Chaudhary, Neha Thakur and Shubham Batra
Affiliation:
- Department of Pharmaceutical Sciences, Chaudhary Bansi Lal University, Bhiwani, 127021, India
Keywords:
Qualitative analysis anti-oxidant, ethanolic extract, oxygen free radicals, ascorbic acid, scavenging activity.
Abstract:
Background: The normal metabolic functioning of aerobic cells is related to free
radical formation. The oxygen utilized in the cell growth gives rise to a number of oxygen free
radicals. Further, these oxygen free radicals interact with lipidic molecules to produce hydroxyperoxides
and various other peroxides also, radicals like superoxide, hydroxyls, and lipoid
peroxides, which lead to cytotoxicity due to their interaction with biological systems. The uncontrolled
generation of free radicals may lead to various diseases and disorders like prostate
cancer, coronary heart disease and also ageing. The therapeutic potential of the plant M. zapota
has been demonstrated in various diseases, such as cancers (e.g., breast, prostate, cervical, and
hepatocellular cancer), diabetes mellitus, arthritis, bacterial infections, and gastrointestinal disorders
(e.g., diarrhea and ulcers) and many medical conditions. The main phytocomponents of
this plant are polyphenols, alkaloids, glycosides, flavonoids, saponins, triterpenoids, carbohydrates,
tannins, and sterols.
Objective: The objective of this study is to investigate qualitative analysis and anti-oxidant potential
of ethanolic extract of Manilkara zapota (L.) P. Royen Leaves. In demand to minimize
the damage caused by free radicals. It is very essential to develop such antioxidants which protect
the body from the effect of free radicals and also do not cause much harm to the human
body. The main phytocomponents of the plant are polyphenols, alkaloids, glycosides, flavonoids,
saponins, triterpenoids, carbohydrates, tannins and sterols which are responsible for antioxidant
activity.
Materials and Methods: Phytochemical screening methods, Gas chromatography–mass spectrometry
(GC-MS), Fourier transform infrared (FTIR), and Hydrogen Peroxide scavenging assay
for analysis of an ethanolic extract of plant leaves.
Results and Discussion: Antioxidant activity was determined by a hydrogen peroxide assay.
Plant extract was examined using phytochemical screening, GC-MS analysis, and FTIR spectra.
The plant extract showed hydrogen peroxide scavenging activity (IC50 = 16.45 μg/ml)
compared to the IC50 values of standard ascorbic acid (IC50 = 9.079 μg/ml).
Conclusion: The present study concluded the antioxidant and phytochemical assessment of the
ethanolic extract of the leaves of Manilkara zapota. The results of the present research study
demonstrated that the antioxidant activity of the plant extract was strong as compared to ascorbic
acid.
