Title:The Differences of Blood Group Typing using Erythrocytes and Leukocytes
Volume: 5
Author(s): Kravchun Pavlo, Leontyeva Frida, Povelichenko Olena and Dielievska Valentyna*
Affiliation:
- Department of Clinical Immunology and Allergology, Named After Academician LT Malaya, Kharkiv National Medical University, Kharkiv, Ukraine
Keywords:
Adsorption, Leukocytes, Erythrocytes, Agglutination, Transplantation, Plasma.
Abstract:
Introduction:
Adsorption and mixed agglutination reactions detect A, B, and H blood group antigens in the cells.
The study aimed to investigate the adsorbing abilities of group-specific polyclonal antibodies using erythrocytes and leukocytes to improve the
quality of blood typing.
Material and Methods:
The adsorption was performed with group B erythrocytes and leukocytes using the usual heated citrated anti-B plasma at 4°C and 37°C. A mixed
agglutination reaction revealed group B antigens on leukocytes and erythrocytes.
Results:
The highest adsorption of antibodies from usual plasma on erythrocytes was observed at 4°C at physiological conditions. Weaker adsorption was
determined at pH 8.0 compared to pH 6.0 (the decrease of agglutination strength from 3+ to 2+ and 1+ accordingly).
The adsorption of polyclonal antibodies on erythrocytes at 37°C was the highest at pH 6.0 and 8.0 compared to physiological conditions (the
decrease of agglutination strength from 3+ to 1+). In contrast, adsorption at 4°C appeared to be stronger at pH 6.0.
The adsorption of IgG antibodies on erythrocytes at 37°C was higher at pH 8.0.
Complete adsorption of IgG antibodies was observed on group B erythrocytes and leukocytes at 37°C and 4°C.
Conclusions:
Adsorption of polyclonal group-specific antibodies on erythrocytes was the highest at 4°C, and adsorption of IgG antibodies was strong at 4°C and
37°C. Adsorption of leukocytes was revealed only with the use of the heated serum. IgG group-specific antibodies from the heated plasma
demonstrated higher adsorbing abilities on leukocytes than IgM antibodies from plasma.