Title:The Effects of Mesenchymal Stem Cells Loaded with Oncolytic
Coxsackievirus A21 on Mouse Models of Colorectal Cancer
Volume: 24
Issue: 9
Author(s): Reza Karbalaee, Saber Mehdizadeh, Hadi Esmaeili Gouvarchin Ghaleh*, Morteza Izadi, Bahman Jalali Kondori, Ruhollah Dorostkar and Seyed Morteza Hosseini
Affiliation:
- Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Keywords:
Anti-tumor immunity, colorectal cancer, mesenchymal stem cells, CT26 cell line, mouse model, Coxsackievirus A21, oncolytic virus.
Abstract:
Background: Cancer is a major cause of death worldwide. Colorectal cancer is the second
most common type. Additional treatments like chemotherapy and radiation therapy may be
recommended. Developing new techniques is vital due to drug resistance and a lack of targeted
therapies.
Objective: In this study, the effects of mesenchymal stem cells (MSCs) loaded with oncolytic Coxsackievirus
A21 (CVA21) on a mouse model of CRC were investigated.
Methods: The therapeutic potency of MSCs loaded with oncolytic CVA21 were evaluated in an
experimental mouse model of colorectal cancer which received an injection CT26 cells per mouse
subcutaneously. Splenocyte proliferation index, lactate dehydrogenase (LDH) assay, nitric oxide
(NO) production assessment, and cytokine assay (IFN-γ, IL-4, IL-10, and TGF-β) in the splenocyte
supernatant were all used to evaluate the impact of MSCs loaded with CVA21.
Results: The results of this study showed that the treatment of a mouse model of colorectal cancer
with MSCs loaded with oncolytic CVA21 could significantly suppress the tumor growth, which
was accompanied by stimulation of splenocytes proliferation index, an increase of NO and LDH.
Also, MSCs loaded with oncolytic CVA21 increased the secretion of IFN-γ and decreased the secretion
of IL-4, IL-10, and TGF-β.
Conclusion: The results of the current study suggest that MSCs loaded with oncolytic CVA21
therapy for the CRC mouse model may have some potential advantages. On the other hand, the results
of the study showed that, in addition to activating the acquired immune system, the use of
MSCs loaded with oncolytic CVA21 also stimulates the innate immune system by increasing level
of nitric oxide.