Title:Characterizing Nanoparticle Isolated by Yam Bean (Pachyrhizus erosus) as a Potential Agent for Nanocosmetics: An in vitro and in vivo Approaches
Volume: 13
Issue: 2
Author(s): Maesa Ranggawati Kusnandar, Indra Wibowo and Anggraini Barlian*
Affiliation:
- School of Life Science and Technology, Institut Teknologi Bandung, Bandung, West Java, Indonesia
- Research Center of Nanoscience and Nanotechnology, Institut Teknologi Bandung, Bandung, West Java, Indonesia
Keywords:
Exosome, plant-derived exosome-like nanoparticle, Pachyrhizus erosus, anti-melanogenic activity, YB-PDENs, analysis.
Abstract:
Background: This study investigated the potential of Plant-Derived Exosome-Like Nanoparticles
(PDENs) as cosmeceutical nanocarriers for treating skin problems, such as scar removal,
face rejuvenation, anti-aging, and anti-pigmentation.
Objectives: Researchers isolated PDENs from Yam Bean ((Pachyrhizus erosus) using PEG-based precipitation,
gradual filtration, and various centrifugations at low temperatures. Followed by in vitro and
in vivo studies using HDF cells and Zebrafish.
Methods: The morphology of the YB-PDENs was determined using TEM analysis, they had a spherical
shape with diameters of 236,83 ± 9,27 nm according to PSA. The study found that YB-PDENs
were stable in aquabidest at 4°C for one month of storage and had ~-26,5 mV of Zeta Potential. The
concentration of YB-PDENs was measured using the BCA Assay, and internalization of YB-PDENs
to HDF cells was observed using a Confocal Laser Scanning Microscope labelled with PKH67.
Results: As for cytotoxicity, after 24 and 72 hours of incubation with YB-PDENs, the viability of
HDF cells remained more than 80%. The study also examined cell migration using the Scratch Assay
and found that at 2,5 μg/mL, YB-PDENs had better migration results than other concentrations. Immunocytochemistry
showed that collagen expression was higher after 14 days of incubation with YBPDENs,
and melanocytes in zebrafish decreased at each concentration compared with controls.
Conclusion: In conclusion, this study is the first to extract and describe PDEN s from Yam Bean
((Pachyrhizus erosus), with YB-PDENs having a promising anti-melanogenic effect in skin treatment.
This study highlights the potential of YB-PDENs as a promising alternative to depigmentation and
skin whitening treatments.
