Title:Protective Assessment of Novel (BNC Formulation) against Brain Tumor
Volume: 20
Issue: 3
Author(s): Anuradha Mishra*, Afza Ahmad, Irfan Ahmad Ansari and Rohit Kumar Tiwari*
Affiliation:
- Amity Institute of Pharmacy, Lucknow, Amity University Uttar Pradesh, Sector-125, Noida-201313, Uttar Pradesh, India
- Department of Clinical
Research, Sharda School of Allied Health Sciences, Sharda University, Gautum Budh Nagar, Uttar Pradesh, 201310, India
Keywords:
Curcuma longa, Bacopa monneiri, Nigella sativa, neuroblastoma, SH-SY5Y, neuroprotective.
Abstract:
Background: Oxidative stress refers to non-homeostatic elevation within intracellular
reactive oxygen species (ROS) levels and is associated with several neuro-related pathological
conditions. Diclofenac is a commonly prescribed non-steroidal anti-inflammatory drug
(NSAID) for treating aches and pain by reducing inflammation. Diclofenac is also associated
with the induction of apoptotic cell death by altering the homeostatic balance within mitochondria.
In the present report, the neuroprotective effects of BNC formulation constituted by Bacopa
monnieri leaves, Nigella sativa and Curcuma longa rhizome seeds were investigated.
Methods: The synthesized formulation was characterized using FT-IR and LC-MS along with
organoleptic evaluation. Thereafter neuroprotective efficacy of BNC formulation was subsequently
investigated against Diclofenac-induced oxidative stress in SH-SY5Y cells. The cells
were pretreated with synthesized formulation and subsequently evaluated for amelioration in
Diclofenac-induced cytotoxicity, and ROS augmentation. The neuroprotective effect of synthesized
formulation was further explored by evaluating the changes in nuclear morphology, and
apoptosis alleviation with concomitant regulatory effects on caspase-3 and -9 activation.
Results: Diclofenac was found to be considerably cytotoxic against human neuroblastoma SHSY5Y
cells. Intriguingly, Diclofenac-mediated toxicity was reduced significantly in SH-SY5Y
cells pretreated with BNC formulation. Augmented ROS levels within Diclofenac-treated SHSY5Y
cells were also reduced in the BNC formulation pretreated SH-SY5Y cells. Furthermore,
BNC formulation pretreated SH-SY5Y cells also exhibited reduced dissipation of mitochondrial
membrane potential, caspase-3 and -9, along with apoptosis after Diclofenac treatment.
Conclusion: These findings indicated that, indeed, Diclofenac induces considerable ROSmediated
apoptosis in SH-SY5Y cells, which further intriguingly ameliorated Diclofenacmediated
cytotoxic effects on SH-SY5Y cells. This manuscript further collected information
about available National and International patents published or granted in preparation of and
thereof applications against motor and non-motor brain dysfunctions.
