Title:Turmeric Extract-loaded Selenium Nanoparticles Counter Doxorubicin-induced
Hepatotoxicity in Mice via Repressing Oxidative Stress, Inflammatory Cytokines, and
Cell Apoptosis
Volume: 24
Issue: 6
Author(s): Barakat M. ALRashdi*, Mohamed M. Hussein, Rawan M. Mohammed, Nada W. Abdelhamed, Maran E. Asaad, Mubarak Alruwaili, Saad M. Alrashidi, Ola A. Habotta, Ahmed E. Abdel Moneim*Shimaa S. Ramadan
Affiliation:
- Department of Biology, College of Science, Jouf University, Sakaka, 72388, Saudi Arabia
- Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt
Keywords:
Nanoselenium, turmeric extract, doxorubicin, hepatotoxicity, apoptosis, oxidative stress.
Abstract:
Background: Doxorubicin (DOX) is an antitumor anthracycline used to treat a variety of malignancies;
however, its clinical use is associated with noticeable hepatotoxicity. Therefore, the current study was designed
to delineate if biosynthesized SeNPs with turmeric extract (Tur-SeNPs) could alleviate DOX-induced
hepatic adverse effects.
Methods: Mice were orally post-treated with Tur extract, Tur-SeNPs, or N-acetyl cysteine after the intraperitoneal
injection of DOX.
Results: Our findings have unveiled a remarkable liver attenuating effect in DOX-injected mice post-treated with
Tur-SeNPs. High serum levels of ALT, AST, ALP, and total bilirubin induced by DOX were significantly decreased
by Tur-SeNPs therapy. Furthermore, Tur-SeNPs counteracted DOX-caused hepatic oxidative stress,
indicated by decreased MDA and NO levels along with elevated levels of SOD, CAT, GPx, GR, GSH, and
mRNA expression levels of Nrf-2. Noteworthily, decreased hepatic IL-1β, TNF-α, and NF-κB p65 levels in addition
to downregulated iNOS gene expression in Tur-SeNPs-treated mice have indicated their potent antiinflammatory
impact. Post-treatment with Tur-SeNPs also mitigated the hepatic apoptosis evoked by DOX injection.
A liver histological examination confirmed the biochemical and molecular findings.
Conclusions: In brief, the outcomes have demonstrated Tur loaded with nanoselenium to successfully mitigate
the liver damage induced by DOX via blocking oxidative stress, and inflammatory and apoptotic signaling.