Title:A Novel Multi-compartment Rotating Bioreactor for Improving ADSC-Spheroid Formation and its Application in Neurogenic Erectile Dysfunction
Volume: 19
Issue: 10
Author(s): Peng Wang, Yang Liu, Xiao-Feng Duan, Xiao-Ying Pan, Xiang-Rui Kong and Yong Yang*
Affiliation:
- Department of Urology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000,
China
- Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun,
China
Keywords:
Centrifugation culture, adipose-derived stem cells (ADSCs), stem cell microspheres, erectile dysfunction, cavernous nerve damage, HUVECs.
Abstract:
Objective: The aim of this study was to construct a multicompartment synchronous rotating
bioreactor (MCSRB) for batch-production of homogenized adipose-derived stem cell (ADSC)
microspheres and treat neurogenic erectile dysfunction (ED).
Methods: Firstly, an MCSRB was constructed using a centrifugal device and hinged trays. Secondly,
influence factors (density, rotational speed) on the formation of ADSC-spheroids were explored.
Finally, a neurogenic ED model was established to verify the effectiveness and safety of
ADSC-spheroids for ED treatment.
Results: An MCSRB promoted ADSCs to gather microspheres, most of which were 90-130 μm in
diameter. Supernatant from three-dimensional culture led to a significant increase in cytokine expression
in ADSCs and migration rate in human umbilical vein endothelial cells (HUVECs) compared
to control groups. The erectile function and pathological changes of the penis were improved
in the ADSC-spheroids treatment group compared to the traditional ADSCs treatment
group (p < 0.01).
Conclusion: Efficient, batch, controlled and homogenized production of ADSC stem cell microspheres,
and effective improvement of erectile dysfunction in neurogenic rats can be achieved using
the MCSRB device.