Title:Protective Effect of Hydroalcoholic Extract of Punica granatum Leaves on
High Fructose Induced Insulin Resistance in Experimental Animals
Volume: 23
Issue: 4
Author(s): Deepti Bandawane*, Ashwini Kotkar and Pooja Ingole
Affiliation:
- Department of Pharmacology, PES’s Modern College of Pharmacy, Nigdi, Pune, India
Keywords:
Insulin resistance, high fructose, oral glucose tolerance, metabolic syndrome, Punica granatum, oxidative stress.
Abstract:
Background: Insulin resistance (IR) is a condition characterized by reduced sensitivity
of body tissues to insulin, leading to impaired regulation of downstream metabolic pathways
and elevated blood glucose levels. Diets rich in fructose have been proven to cause insulin resistance
in test rats, resulting in decreased insulin sensitivity, particularly in the liver, and compromised
disposal of glucose from the body. In the search for effective treatments, Plant-derived
formulations have gained popularity because to their ability for treating a variety of ailments.
One such plant is Punica granatum Linn. from the Punicaceae family, which has long been used
in the treatment of diabetes and its consequences. This study investigates the insulin-resistant
activity of an extract from Punica granatum leaves. The study goal is to assess the possible protective
role of Punica granatum against insulin resistance through various analyses, including
serum glucose and insulin levels, lipid profile assessment, measurement of liver enzymes (ALP,
SGOT, SGPT), and histopathological examination of liver sections.
Methods: The study involves several key methods to evaluate the insulin-resistant activity of
Punica granatum extract in high fructose diet induced insulin resistance animal model. The extract
was administered orally to the experimental animals. These methods include the measurement
of serum glucose and serum insulin levels, analysis of the lipid profile, quantification of
liver enzymes such as ALP, SGOT, and SGPT, and a detailed histopathological examination of
liver tissue sections. These analyses collectively provide insights into the impact of Punica
granatum extract on insulin resistance and related metabolic parameters.
Results: Findings of this study provide insight on the possible benefits of Punica granatum extract
on insulin resistance. Through the assessment of serum glucose and insulin levels, lipid profile
analysis, and measurement of liver enzymes, the study elucidates the impact of the extract on
key metabolic indicators. Additionally, the histopathological examination of liver sections provides
visual insights into the structural changes that may occur as a result of the treatment.
Conclusion: In conclusion, this study highlights the ability of Punica granatum extract as a candidate
for addressing insulin resistance. The findings suggest that the extract may have a protective
role against insulin resistance, as evidenced by improvements in serum glucose and insulin
levels, lipid profile, liver enzyme levels, and histopathological characteristics. Further research
and investigations are warranted to fully understand the mechanisms underlying these observed
effects and to validate the potential of Punica granatum extract as a therapeutic option for managing
insulin resistance and its associated complications.