Title:Comparative Study of Liposomal and Ethosomal Formulations of Curcuma heyneana Rhizome Extract in a Transdermal Delivery System
Volume: 13
Issue: 2
Author(s): Idha Kusumawati*, Kresma Oky Kurniawan, Rohmania Rohmania, Bernasdito Ade Pratama, Yusuf Alif Pratama, Subhan Rullyansyah, Mega Ferdina Warsito, Retno Widyowati, Eka Pramyrtha Hestianah and Katsuyoshi Matsunami
Affiliation:
- Department of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building,
Surabaya, 60155, Indonesia
- Natural Product Drug Discovery and Development Research Group, Faculty of
Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building, Surabaya, 60155, Indonesia
Keywords:
Curcuma heyneana, transdermal delivery, phospholipid, ethosome, liposome, penetration, medicine.
Abstract:
Aims: This study aimed to develop an anti-aging nanoformulation with Curcuma heyneana
extract as bioactive substance.
Background: Curcuma heyneana Valeton & Zipj extract has been proven in previous research to
have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a
potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need
to penetrate into deeper skin layers to ensure effectivity. Thus, a transdermal delivery system is
needed to deliver the extract to a deeper skin layer.
Objectives: The objective of the study was to compare the permeability and anti-ageing activity of
liposomal and ethosomal formulations of C. heynena rhizome ethanolic extract.
Methods: In this study, C. heyneana extract was loaded into a phospholipid vesicular system in the
form of liposome and ethosome formulations using the ethanolic injection method. The anti-ageing
activity was assessed by analyzing the epidermal thickness, number of sunburn cells, distance
between collagen fibers, and number of fibroblasts. While the histologic specimen scoring was
carried out for the in vivo penetration study.
Results: The ethosomal formulation had been found to have better penetration ability since it was
able to reach the lower dermis area compared to the liposomes, which only reached the upper dermis.
The ethosomal formulation of C. heyneana extract exhibited a better anti-ageing activity based on
the parameters of epidermal thickness, sunburn cell count, fibroblast count, and the distance between
collagen fibres in rat skin histology.
Conclusion: Ethosomes have been found to be a more proficient carrier system for transdermal delivery
of C. heyneana extract compared to liposomes. Meanwhile, their penetration correlated with
the effectivity of the formulation, suggesting that the vesicular system enhanced the penetration ability
of the extract.