Title:Enrichment of Isaria felina Culture with Selenium Enhances its in vivo Antitumor
Effects on H22 Hepatoma via Decreasing the Expression of VEGF
Volume: 23
Issue: 14
Author(s): Xihua Yang*, Yongming Yang, Lili Zhao, Lixia Chen, Jing Wang, Lei Yan and Jinfeng Ma*
Affiliation:
- Shanxi Provincial Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer
Hospital Affiliated to Shanxi Medical University, 030013, Taiyuan, Shanxi, China
- Department of General Surgery, Shanxi Provincial
Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to
Shanxi Medical University, 030013, Taiyuan, Shanxi, China
Keywords:
Anti-tumor effects, hepatoma cells, Isaria felina, selenium-enriched culture, interleukin 2, vascular endothelial growth factor.
Abstract:
Background: The polysaccharide extract of C. sinensis, Isaria felina (IF), has antitumor effects. Selenium
(Se) can improve disease prevention and reduce the toxicity of toxic elements, but the effect of Se-enriched IF on hepatoma
remains unknown.
Objective: To determine the organic transformation of Se and compare the antitumor effects between Se-enriched IF
(IF-Se) and IF on xenograft H22 hepatoma-bearing mice.
Methods: Se was added to the solid-state culture medium, and the organic Se content was detected by HPLC-ICP-MS.
Forty-two Kunming mice were randomly divided into seven groups to test the antitumor effects of low- (300 mg/kg)
and high- (600 mg/kg) doses of IF-Se and IF through xenograft. Huai’er granules were administered as the positive
control. In addition, interleukin (IL)-2 and vascular endothelial growth factor (VEGF) expressions were measured by
enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry method.
Results: The conversion rate in the IF-Se70, IF-Se140, and IF-Se280 groups were 91.5%, 93.4%, and 89.3%, respectively.
Therefore, IF-Se140 was used to carry out the subsequent experiments. The tumor inhibition rates of IF-Se were
significantly higher compared with IF (P < 0.05). Moreover, the spleen coefficient, IL-2, and VEGF expression levels
significantly decreased (all Ps < 0.05), and the thymus coefficient significantly increased (P < 0.05) in the high-dose
IF-Se group compared with the model control group.
Conclusion: The inhibitory effects of IF on H22 hepatoma-bearing mice were enhanced after Se enrichment. Therefore,
Se-enriched IF might be a new strategy for treating hepatoma.
