Generic placeholder image

Infectious Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

Research Article

A Study of Antibacterial Effect of Nigella sativa Seed Extracts on Bacterial Isolates from Cases of Wound Infection

Author(s): Boinu Babu, Pooja Rao, Ethel Suman and Jeppu Udayalaxmi*

Volume 23, Issue 5, 2023

Published on: 28 April, 2023

Article ID: e030423215400 Pages: 5

DOI: 10.2174/1871526523666230403095441

Price: $65

Abstract

Background: With an increasing trend of pathogenic bacteria developing resistance to the existing drugs, there is a need for newer therapeutic measures. Nigella sativa seeds and oil have been used for decades as Ayurveda, Unani Tibb and other forms of traditional medicine for various disorders. Thymoquinone is one of the active components of the N. sativa seeds.

Objective: The present study determines the antibacterial effect of crude methanolic extract N. sativa seeds and thymoquinone against bacteria causing wound infection.

Methods: Samples obtained from cases of wound infection received at a Microbiology laboratory attached to a tertiary care hospital over a period of six months were included in the study. The antibacterial effect of crude methanolic extract of N. sativa seeds was determined by the Punch Well method. The minimum inhibitory concentration (MIC) of thymoquinone against bacteria isolated from cases of wound infection was determined by the Micro Broth Dilution technique.

Results: A total of 60 isolates were collected from 60 samples of wound infection. By the Punch Well method, Staphylococcus aureus showed varying zones of inhibition whereas all gram-negative bacilli and Enterococcus faecalis did not show any zone of inhibition. Thymoquinone showed good antibacterial activity against S. aureus with MIC values ranging from 2-8μg/ml for most of the isolates. Uniformly, MIC of thymoquinone against all gram-negative bacilli and E. faecalis was >128μg/ml, p<0.001. It was found that methicillin-resistant S. aureus (MRSA) isolates showed higher MIC than methicillin sensitive S. aureus (MSSA) isolates p<0.05.

Conclusion: Antibacterial activity of thymoquinone was very good against S. aureus but showed limited activity against Enterobacteriaceae members and E. faecalis isolated from patients with wound infection. Thymoquinone may be considered a potential antibacterial agent against wound infection caused by S. aureus.

Keywords: Extract, Enterobacteriaceae, Enterococcus faecalis, minimum inhibitory concentration, MRSA, Staphylococcal aureus, thymoquinone, wound infection.

Graphical Abstract
[1]
Forouzanfar F, Bazzaz BS, Hosseinzadeh H. Black cumin (Nigella sativa) and its constituent (thymoquinone): A review on antimicrobial effects. Iran J Basic Med Sci 2014; 17(12): 929-38.
[PMID: 25859296]
[2]
Yimer EM, Tuem KB, Karim A. Nigella sativa L. (Black Cumin): A promising natural remedy for wide range of illnesses. Evid Based Complement Alternat Med 2019; 2019: 1528635.
[http://dx.doi.org/10.1155/2019/1528635]
[3]
Al-Ameedy TH, Omran R. Antimicrobial activity of Nigella sativa extract against some bacterial and fungal species. JUBPAS 2019; 27(1): 277-86.
[4]
Abraham AO, Abdulazeez AK, Seun OO, Ogonna DW. Antimicrobial activity of N-Hexane extract of Nigella sativa against some pathogenic bacteria. Am J Biomed Res 2019; 6(5): 430-4.
[http://dx.doi.org/10.34297/AJBSR.2019.06.001077]
[5]
Derbal S, Niar A. Antibacterial activity of honey and Nigella sativa L. seed extracts against animal wound bacteria. Int J Vet Sci Res 2019; 5(1): 30-4.
[6]
Mohamed MSN, Jaikumar K, Babu A, Anand D, Saravanan P. A study on the in vitro antifungal activity of Nigella sativa (Linn.) seed extract and it’s phytochemical screening using Gc-Ms analysis. World J Pharm Pharm Sci 2015; 4(8): 1003-11.
[7]
Shah SB, Ali Shah SI, Sartaj L, Zaman S, Bilal M, Salman M. Effect of Nigella sativa seeds extracts on clinically important bacterial and fungal species. MOJ Bioequivalence Bioavailability 2018; 5(4): 218-21.
[http://dx.doi.org/10.15406/mojbb.2018.05.00105]
[8]
Kamal A, Ahmad IZ. Alteration in antibacterial potential of Nigella sativa L. seed during different phases of germination. Int J Curr Microbiol Appl Sci 2014; 3(3): 268-82.
[9]
Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 2011; 11(1): 29.
[http://dx.doi.org/10.1186/1472-6882-11-29] [PMID: 21489272]
[10]
Halawani E. Antibacterial activity of thymoquinone and thymohydroquinone of Nigella sativa L. and their interaction with some antibiotics. Adv Biol Res (Faisalabad) 2009; 3(5): 148-52.
[11]
Al-Khalifa KS, Al-Sheikh R, Al-Hariri MT, et al. Evaluation of the antimicrobial effect of thymoquinone against different dental pathogens: An in vitro study. Molecules 2021; 26: 6451.
[http://dx.doi.org/10.3390/molecules26216451]
[12]
CLSI, Methods for Dilution Antimicrobial Susceptibility Tests forBacteria That Grow Aerobically; Approved Standard- Tenth Edition.CLSI document M07-A 10. Wayne, PA: Clinical and Laboratory Standard Institute 2015; pp. 27-9.
[13]
Rondevaldova J, Novy P, Urban J, Kokoska L. Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmat™ as a vapor barrier. Arab J Chem 2017; 10(4): 566-72.
[http://dx.doi.org/10.1016/j.arabjc.2015.04.021]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy