Title:Hsa_Circ_0000021 Sponges miR-3940-3p/KPNA2 Expression to Promote
Cervical Cancer Progression
Volume: 17
Author(s): Qingyuan Zeng, Kun Feng, Yang Yu and Yumei Lv*
Affiliation:
- Department of Gynecology and Obstetrics, General Hospital of Western Theater Command of the Chinese People's Liberation Army, No. 270
Rongdu Avenue, Jinniu District, Chengdu 610083, Sichuan, China
Keywords:
Hsa_circ_0000021, CC, MiR-3940-3p, KPNA2, CircRNAs, Genetics.
Abstract:
Background:
Circular RNAs (circRNAs) have a vital role in the occurrence of numerous cancers. However, its function and pattern of expression in cervical
cancer (CC) remain unclear. This research aims to investigate the hsa_circ_000002’s regulatory mechanism in CC.
Methods:
Hsa_circ_0000021, miR-3940-3p, and KPNA2 expression levels were estimated through qRT-PCR. Nuclear/cytoplasmic separation was
conducted to find the subcellular location of hsa_circ_0000021. Western blot was done to estimate the levels of KPNA2 protein. CCK-8, BrdU,
wound healing, transwell, and tumor xenograft assays were performed to study how hsa_circ_0000021/miR-3940-3P/KPNA2 function affect CC.
Hsa_circ_0000021’s targeting relationships with miR-3940-3p and KPNA2 were ascertained through RIP and luciferase experiments.
Results:
Hsa_circ_0000021 and KPNA2 were overexpressed and inversely associated with the levels of miR-3940-3p in CC. Knocking down either
hsa_circ_0000021 or KPNA2 repressed the growth of CC tumors as well as the proliferation, invasion, and migration of CC cells. Silencing
miR-3940-3p promoted the malignant proliferation of CC cells. Regarding its mechanism, hsa_circ_0000021 affected the malignant CC cell
proliferation via the sponging of miR-3940-3p, which targeted KPNA2.
Conclusion:
Hsa_circ_0000021 regulates the miR-3940-3p/KPNA2 axis to promote CC occurrence. This potentially is a novel target for CC treatment.
