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Current Genomics

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ISSN (Print): 1389-2029
ISSN (Online): 1875-5488

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Research Article

Chromosome-level Genome Assembly and Sex-specific Differential Transcriptome of the White-backed Planthopper, Sogatella furcifera

Author(s): Yu-Xuan Ye, Dan-Ting Li, Si-Yu Zhang, Zhi-Cheng Shen and Chuan-Xi Zhang*

Volume 23, Issue 6, 2022

Published on: 17 January, 2023

Page: [400 - 411] Pages: 12

DOI: 10.2174/1389202924666230102092822

open access plus

Abstract

Background: The white-backed planthopper (WBPH), Sogatella furcifera, causes great damage to many crops (mainly rice) by direct feeding or transmitting plant viruses. The previous genome assembly was generated by second-generation sequencing technologies, with a contig N50 of only 51.5 kb, and contained a lot of heterozygous sequences.

Methods: We utilized third-generation sequencing technologies and Hi-C data to generate a highquality chromosome-level assembly. We also provide a large amount of transcriptome data for fulllength transcriptome analysis and gender differential expression analysis.

Results: The final assembly comprised 56.38 Mb, with a contig N50 of 2.20 Mb and a scaffold N50 of 45.25 Mb. Fourteen autosomes and one X chromosome were identified. More than 99.5% of the assembled bases located on the 15 chromosomes. 95.9% of the complete BUSCO Hemiptera genes were detected in the final assembly and 16,880 genes were annotated. 722 genes were relatively highly expressed in males, while 60 in the females.

Conclusion: The integrated genome, definite sex chromosomes, comprehensive transcriptome profiles, high efficiency of RNA interference and short life cycle substantially made WBPH an efficient research object for functional genomics.

Keywords: Chromosome-level genome assembly, White-backed planthopper (WBPH), Sogatella furcifera, BUSCO, RNA interference, sex chromosomes.

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