Title:TLR3 Polymorphisms in HIV-infected Individuals Naïve to ART
Volume: 20
Issue: 5
Author(s): HariOm Singh*Dharmesh Samani
Affiliation:
- Department of Molecular Biology, ICMR-National AIDS Research Institute, Pune, India
Keywords:
TLR3, genetic polymorphism, host genetic factor, HIV infected individuals, haplotype, ELISA.
Abstract:
Background: TLR3 polymorphisms affect the risk of HIV infection and modify the disease
course. Consequently, we analyzed the association of TLR3 polymorphism (rs5743312,
rs3775296, and rs3775291) with susceptibility to HIV-1 acquisition and disease progression.
Methods: This is a cross-sectional study. Genotyping of TLR3 polymorphisms is completed by utilizing
the PCR-RFLP technique in 153 HIV naive subjects and 158 healthy controls.
Results: A haplotype is a physical grouping of genomic variants that tend to be inherited together.
The TCC haplotype was increased in HIV-infected individuals compared with healthy controls
(0.05% versus 0.03%). TLR3 rs3775291CT genotype was associated with the early stage of HIV infection
(OR = 2.19, P = 0.04), with a higher occurrence in the advanced stage of HIV infection
when contrasted with healthy controls (41.2% versus 32.3%). TLR3 rs3775296 CA genotype was
likely to be associated with the intermediate stage of HIV infection (19.5% versus 31.6%, OR =
0.42, P = 0.06). TLR3 rs5743312TT genotype was more prevalent in the advanced stage of HIV infection
compared with healthy controls (2.9% versus 1.9%). TLR3 rs3775296CA genotype was less
prevalent in HIV subjects devouring tobacco when contrasted with non-users (9.1% versus 34.9%,
OR = 0.25, P = 0.09). TLR3 rs3775296AA and rs3775291CT and TT genotypes have been
overrepresented in HIV subjects using alcohol when contrasted with non-users (5.6% versus 1.1%,
OR = 1.83, P = 0.67; 50.0% versus 42.2%, OR = 1.84, P = 0.31; 5.6% versus 3.3%, OR = 2.70, P
= 0.50). In the multivariate examination, the rs5743312TT genotype showed a greater risk for HIV
infection (OR = 1.86, P = 0.50).
Conclusion: TLR3 rs3775291 C/T polymorphism may assist the risk of disease progression in alcohol
consumers. TLR3 rs3775291 CT genotype may enhance the disease progression, whereas the
TLR3 rs3775296 CA genotype may protect against disease progression.