Title:FOXN3 Expression Regulated by miR-299-5p Inhibiting the Proliferation,
Migration and Invasion of Oral Squamous Cell Carcinoma Cells
Volume: 29
Issue: 9
Author(s): Kamilijiang Maimaitiming, Alimire Yilihamu, Kudusi Keyimu, Rexiati Keranmu, Jun Li, Hui Xu and Duolikun Wufuer*
Affiliation:
- Department of Maxillofacial Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang, China
Keywords:
Oral squamous cell carcinoma, proliferation, metastasis, miR-299-5p, FOXN3, cell lines.
Abstract:
Background: Oral squamous cell carcinoma (OSCC) is one of the commonest
malignancies of the oral cavity. FOXN3 is a tumor suppressor that represses the progression of
many tumors. Nonetheless, its role in OSCC has not been elucidated. This work is performed to
probe the role and dysregulation mechanism of FOXN3 in OSCC.
Methods: FOXN3 mRNA and miR-299-5p expressions were quantified by quantitative real-time
polymerase chain reaction (qRT-PCR); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay was employed to detect OSCC cell growth; Transwell experiment was
conducted to detect cell migration and invasion; dual-luciferase reporter experiment and
bioinformatics were adopted to analyze the relationship between miR-299-5p and FOXN3; Western
blot was implemented to detect FOXN3 protein expression.
Results: FOXN3 expression was remarkably down-modulated, and miR-299-5p expression was
markedly up-modulated in OSCC tissues and cell lines compared with paracancerous tissues and
normal oral epithelial cell line. FOXN3 overexpression impeded OSCC cell growth, migration and
invasion. FOXN3 was proven to be a downstream target of miR-299-5p, and miR-299-5p mimics
enhanced OSCC cell growth, migration and invasion. Moreover, FOXN3 overexpression partially
reversed the promoting effects of miR-299-5p mimics on OSCC cell growth, migration and invasion.
Conclusion: FOXN3 expression is remarkably down-modulated in OSCC tissues and cell lines, and
miR-299-5p targets FOXN3 to facilitate OSCC cell growth, migration and invasion. These results
imply that miR-299-5p/FOXN3 axis may be a potential target for OSCC treatment.