Generic placeholder image

Anti-Infective Agents


ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

Research Article

Antibacterial Activity of Pulicaria odora Leaf Phenolics Collected in Algeria

Author(s): Karima Saidani, Naima Touati*, Yasmina Hassaini, Amel Kasri and Fatiha Bedjou

Volume 21, Issue 1, 2023

Published on: 13 May, 2022

Article ID: e010522204321 Pages: 6

DOI: 10.2174/2211352520666220501163744

Price: $65


Background: Pulicaria is a genus of the Asteraceae family, tribe Inuleae, containing 100 species with a distribution from Europe to North Africa to Asia.

Objective: In this work, the study is focused on the difference in phytochemical and antibacterial activity of Pulicaria odora harvested in two regions of northern Algeria.

Methods: Two maceration extraction methods were used; a stir plate at room temperature and bath shaker at 37 °C. Total phenolics and flavonoids were carried out using Folin-Ciocalteu and aluminium chloride methods, respectively. Antibacterial activity was evaluated by agar diffusion method on four bacterial strains Listeria innocua, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.

Results: The results found showed that the highest total polyphenol content was 271,8 ± 22, 52 mg gallic acid equivalent/g dry matter (mg GAE/g of DM); the best flavonoid content is 51,53 ± 4,42 mg quercetin equivalent / g of dry matter (mg QE/g of DM) harvested in Bejaia. The best levels of total polyphenols and flavonoids from the plant harvested in the region of Tizi-Ouzou are 218,23 ± 12.33 mg GAE / g of DM, 54,15 ± 5,98 mg QE/ g DM respectively. The two phenolic extracts obtained by the two solvents at a concentration of 70% are more active against S. aureus (17.66 mm for the sample of Bejaia at the concentration of 1mg /20μL), while a moderate sensitivity is recorded against other strains for the two samples.

Conclusion: Pulicaria odora leaf extract is strong against bacterial infection and can be used as medicinal remedy and in food industry.

Keywords: Asteraceae, antibacterial activity, flavonoids, phytochemical, Pulicaria odora, total polyphenols.

Graphical Abstract
Bouzid, W.; Yahia, M.; Abdeddaim, M.; Aberkane, M.; Ayachi, A. Evaluation de l’activité antioxydante et antimicrobienne des extraits de l’Aubepine Monogyne. Leban. Sci. J., 2011, 12(1), 59.
Keita, Y.; Koné, O.; Ly, A.K.; Häkkinen, V. Étude chimique et de l’activité antibactérienne des distillats de quelques variétés de mangue de Guinée. C. R. Chim., 2004, 7(10), 1095-1100.
Karou, D.; Dicko, M.H.; Simpore, J.; Traore, A.S. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. Afr. J. Biotechnol., 2005, 4(8), 823-828.
Hammerschmidt, R. Phenols and plant–pathogen interactions: The saga continues. Physiol. Mol. Plant Pathol., 2005, 66(3), 77-78.
Cowan, M.M. Plant products as antimicrobial agents. Clin. Microbiol. Rev., 1999, 12(4), 564-582.
[] [PMID: 10515903]
Quezel, P.; Santa, S. New flora of Algeria and southern desert regions; Cent. Natn. Rech. Scient: Paris, 1962, p. 1170.
Ezoubeiri, A.; Gadhi, C.A.; Fdil, N.; Benharref, A.; Jana, M.; Vanhaelen, M. Isolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L. J. Ethnopharmacol., 2005, 99(2), 287-292.
[] [PMID: 15894140]
Bammou, M.; Bouhlali, E.D.T.; Sellam, K.; Ramchoun, M.; McDonald, A.G.; Ouzidan, Y.; Ibijbijen, J.; El Rhaffari, L.; Nassiri, L. Investigation on chemical composition, antioxidant and antimicrobial potential of Pulicaria mauritanica essential oil applied by direct addition or vapor contact. American J. Essential Oils Natural Products, 2019, 7(1), 7-13.
Al-Hajj, N.Q.M.; Ma, C.; Thabit, R.; Al-alfarga, A.; Gasmalla, M.A.; Musa, A.; Aboshora, W.; Wang, H. Chemical composition of essential oil and mineral contents of Pulicaria inuloides. J. Acad. Ind. Res., 2014, 2(12), 675.
Lougraimzi, H.; Soufiane, M.S.; Bouaichi, A. QOTBA, I. Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil. Natural Volatiles Essential Oils, 2020, 7(2), 35-43.
Touati, N.; Saidani, K.; Boudries, H.; Hammiche, H.; Ouazene, N.; Bedjou, F. Antibacterial activity of phenolic compounds of Pulicaria odora, wild plant in northern Algeria. Int. Food Res. J., 2018, 25(5), 2021-2030.
Mohamed, E.A.A.; Muddathir, A.M.; Osman, M.A. Antimicrobial activity, phytochemical screening of crude extracts, and essential oils constituents of two Pulicaria spp. growing in Sudan. Sci. Rep., 2020, 10(1), 17148.
[] [PMID: 33051571]
Williams, C.A.; Harborne, J.B.; Greenham, J.R.; Grayer, R.J.; Kite, G.C.; Eagles, J. Variations in lipophilic and vacuolar flavonoids among European Pulicaria species. Phytochemistry, 2003, 64(1), 275-283.
[] [PMID: 12946426]
Djeridane, A.; Yousfi, M.; Nadjemi, B.; Boutassouna, D.; Stocker, P.; Vidal, N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem., 2006, 97(4), 654-660.
Tawaha, K.; Alali, F.Q.; Gharaibeh, M.; Mohammad, M.; El-Elimat, T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem., 2007, 104(4), 1372-1378.
Škerget, M.; Kotnik, P.; Hadolin, M.; Hraš, A.R.; Simonič, M.; Knez, Ž. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem., 2005, 89(2), 191-198.
Quettier-Deleu, C.; Gressier, B.; Vasseur, J.; Dine, T.; Brunet, C.; Luyckx, M.; Cazin, M.; Cazin, J-C.; Bailleul, F.; Trotin, F. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharmacol., 2000, 72(1-2), 35-42.
[] [PMID: 10967451]
Suay, I.; Arenal, F.; Asensio, F.J.; Basilio, A.; Cabello, M.A.; Díez, M.T.; García, J.B.; del Val, A.G.; Gorrochategui, J.; Hernández, P.; Peláez, F.; Vicente, M.F. Screening of basidiomycetes for antimicrobial activities. Antonie van Leeuwenhoek, 2000, 78(2), 129-139.
[] [PMID: 11204765]
Tuncel, G.; Nergiz, C. Antimicrobial effect of some olive phenols in a laboratory medium. Lett. Appl. Microbiol., 1993, 17(6), 300-302.
Moroh, J.; Bahi, C.; Dje, K.; Loukou, Y.; Guede-Guina, F. Étude de l’activité antibactérienne de l’extrait acétatique (EAC) de Morinda morindoides (Baker) milne-redheat (rubiaceae) sur la croissance in-vitro des souches d’Escherichia coli. Bull. Soc. R. Sci. Liege, 2008, 77, 44-61.
Hayouni, E.A.; Abedrabba, M.; Bouix, M.; Hamdi, M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem., 2007, 105(3), 1126-1134.
Zhao, X.; Xue, C-H.; Li, B-F. Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica. J. Appl. Phycol., 2007, 20(4), 431-436.
Spigno, G.; Tramelli, L.; De Faveri, D.M. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J. Food Eng., 2007, 81(1), 200-208.
Hendra, R.; Ahmad, S.; Sukari, A.; Shukor, M.Y.; Oskoueian, E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int. J. Mol. Sci., 2011, 12(6), 3422-3431.
[] [PMID: 21747685]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy