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General Review Article

A Review on CRISPR-mediated Epigenome Editing: A Future Directive for Therapeutic Management of Cancer

Author(s): Rudra Chakravarti, Swadhin Kumar Lenka, Anupam Gautam, Rajveer Singh, Velayutham Ravichandiran, Syamal Roy and Dipanjan Ghosh*

Volume 23, Issue 8, 2022

Published on: 14 February, 2022

Page: [836 - 853] Pages: 18

DOI: 10.2174/1389450123666220117105531

Price: $65

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Abstract

Recent studies have shed light on the role of epigenetic marks in certain diseases like cancer, type II diabetes mellitus (T2DM), obesity, and cardiovascular dysfunction, to name a few. Epigenetic marks like DNA methylation and histone acetylation are randomly altered in the disease state. It has been seen that methylation of DNA and histones can result in down-regulation of gene expression, whereas histone acetylation, ubiquitination, and phosphorylation are linked to enhanced expression of genes. How can we precisely target such epigenetic aberrations to prevent the advent of diseases? The answer lies in the amalgamation of the efficient genome editing technique, CRISPR, with certain effector molecules that can alter the status of epigenetic marks as well as employ certain transcriptional activators or repressors. In this review, we have discussed the rationale of epigenetic editing as a therapeutic strategy and how CRISPR-Cas9 technology coupled with epigenetic effector tags can efficiently edit epigenetic targets. In the later part, we have discussed how certain epigenetic effectors are tagged with dCas9 to elicit epigenetic changes in cancer. Increased interest in exploring the epigenetic background of cancer and non-communicable diseases like type II diabetes mellitus and obesity accompanied with technological breakthroughs has made it possible to perform large-scale epigenome studies.

Keywords: Epigenetics, CRISPR, dCas9, epieffector, epigenetic marks, DeepCRISPR, histone modification, DNA methylation.

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Graphical Abstract

Graphical abstract illustrating key findings of the study

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