Title:Development of an Insulin Nano-delivery System through Buccal Administration
Volume: 19
Issue: 8
Author(s): Diaa Al-Domi, Ayat Bozeya*Mohamed Al-Fandi
Affiliation:
- Institute of Nanotechnology, Jordan University of Science and Technology, Irbid 22110, Jordan
Keywords:
Insulin, buccal administration, chitosan, poly (lactic-co-glycolic acid), bioadhesion, w-o-w microemulsion, nanoparticles.
Abstract:
Aim: The aim of the study was to develop a new nano-delivery system for buccal administration
of insulin.
Background: Biodegradable polymeric nanoparticles (PNPs) had undergone countless breakthroughs
in drug delivery systems. The main objective of PNPs application in delivering and carrying
different promising drugs is to make sure that the drugs are being delivered to their action sites,
maximizing the desired effect and overcoming their limitations and drawbacks.
Objective: The main goals of this study were to produce an insulin consumable nano-delivery system
for buccal administration and enhance the mucoadhesive effect in sustaining insulin release.
Methods: Water-oil-water (W-O-W) microemulsion solvent evaporation technique was used for
the preparation of nanoparticles consisting of positively charged poly (D, L-lactide-co-glycolide)
coated with chitosan and loaded with insulin. Later, a consumable buccal film was prepared by the
spin coating method and loaded with the previously prepared nanoparticles.
Results: The newly prepared nanoparticle was assessed in terms of size, charge and surface morphology
using a Scanning Electron Microscope (SEM), zeta potential, Atomic Force Microscope
(AFM), and Fourier Transform Infra-red (FTIR) spectroscopy. An in vitro investigation of the insulin
release from nanoparticles and buccal film demonstrated controlled as well as sustained delivery
over 6 hrs. The cumulative insulin release decreased to about 28.9% with buccal film compared
to the nanoparticle (50%).
Conclusion: The buccal film acted as a barrier for insulin release. Therefore, the release was sustained.
