Title:Investigation of Antioxidant Activity and Analysis of Phenolic Compounds of some Asteraceae Plants by HPLC: A Comparison Between Methanol and Ethanol Extracts
Volume: 2
Author(s): Donia Anvari*Rashid Jamei
Affiliation:
- Department of Biology, Faculty of Science, Urmia University, Serow Road, Urmia,Iran
Keywords:
Antioxidant activity, phenolic compounds, flavonoid, radical scavenging activity, asteraceae, correlation, HPLC.
Abstract:
Background: Medicinal and healing plants have been used in treating human diseases
for centuries because of their therapeutic effects. They may assist in curing common ailments such
as a multitude of skin problems, various disorders from muscle spasms to cuts and wounds. They
can be used to relieve symptoms of different illnesses from cold to some forms of arthritis or some
allergies as well. The Asteraceae plant is a strong source of antimicrobial and antioxidant agents
and this paper focuses on its specifications.
Objectives: The specific aim of this study was to investigate the antioxidant potential and radical
scavenging of different solvents (Methanol and Ethanol) of five species i.e.Artemisia absinthium
L., Arctium lappa L., Centaurea cyanus L., Silybum marianum L., and Echinops ritro L. belonging
to the Asteraceae family.
Methods: Methanol and ethanol extracts of the above plants were prepared. The obtained extracts
were evaluated for total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity
(TAC), chain-breaking activity (CBA), thiobarbituric acid (TBA) and 2, 2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging activity. Also, Ferric reducing antioxidant power (FRAP), Nitric
oxide (NO) radical scavenging, Hydrogen peroxide (H2O2) radical scavenging and Superoxide
(O2
-) radical inhibition assay were measured. Phenolic compounds were determined and measured
by high-performance liquid chromatography (HPLC) as well.
Results: The collected and analyzed data showed that the highest values for the TPC, TFC, TBA,
and DPPH were related to methanol extract of A. lappa L. Moreover, maximum values for the
CBA, H2O2 and O2
- were observed in the ethanol extract of E. ritro L., while methanol extract of E.
ritro L. showed the highest amount of FRAP and NO. Eventually, the highest value for TAC was
related to A. absintium L. and it was also realized that methanol compared to ethanol solvent was
more successful in the extraction procedure.
Conclusions: These findings suggest that A. lappa L. and E. ritro L. extracts can be considered as
excellent natural antioxidant agents and the type of the solvent can affect the extraction of phenolic
compounds. Sinapic acid as the highest level of phenolic acid was found in S. marianum L.
