Aim and Objective: Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spreads in humans through breathing resulting in an indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted.
Materials and Methods: Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T – cell-based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin.
Results: Two epitopes, SPVIAEHYY and LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol and 0.15 kcal/mol with HLA- B*35:03 and HLA- DRB1 * 07:03, respectively by using allele - Class I and Class II from AutoDock. These two peptides predicted by the reverse vaccinology approach are likely to induce immune response mediated by T – cells.
Conclusion: Simulation using GROMACS has revealed that LVNDGLNII epitope forms a more stable complex with HLA molecule and will be useful in developing the epitope-based Nipah virus vaccine.
[http://dx.doi.org/10.1080/01652176.2019.1580827 ] [PMID: 31006350]
[http://dx.doi.org/10.3201/eid1307.061128 ] [PMID: 18214175]
[http://dx.doi.org/10.1126/science.288.5470.1432 ] [PMID: 10827955]
[http://dx.doi.org/10.1128/JVI.78.11.5633-5641.2004 ] [PMID: 15140960]
[http://dx.doi.org/10.1371/journal.pone.0012709 ] [PMID: 20856799]
[http://dx.doi.org/10.1371/journal.pone.0047790 ] [PMID: 23094089]
[http://dx.doi.org/10.1128/JVI.00215-16 ] [PMID: 27147733]
[http://dx.doi.org/10.1038/s41598-019-53037-0 ] [PMID: 31723221]
[http://dx.doi.org/10.1093/nar/gki070 ] [PMID: 15608167]
[http://dx.doi.org/10.4049/jimmunol.1700893 ] [PMID: 28978689]
[http://dx.doi.org/10.1093/nar/gku938 ] [PMID: 25300482]
[http://dx.doi.org/10.1016/j.apjtm.2017.06.016 ] [PMID: 28756915]
[http://dx.doi.org/10.1080/07391102.2019.1667265 ] [PMID: 31514687]
[http://dx.doi.org/10.1093/cid/ciz010 ] [PMID: 30615097]
[http://dx.doi.org/10.3201/eid2506.181620 ] [PMID: 31107231]
[http://dx.doi.org/10.1016/j.antiviral.2013.06.012 ] [PMID: 23838047]
[http://dx.doi.org/10.1038/srep30916 ] [PMID: 27484128]
[http://dx.doi.org/10.1038/s41594-019-0308-9 ] [PMID: 31570878]