Title:Anti-Cancerous Effect of Rutin Against HPV-C33A Cervical Cancer Cells via G0/G1 Cell Cycle Arrest and Apoptotic Induction
Volume: 20
Issue: 3
Author(s): Fahad Khan, Pratibha Pandey*, Tarun K. Upadhyay, Asif Jafri, Niraj K. Jha, Rashmi Mishra and Vineeta Singh
Affiliation:
- Department of Biotechnology, Noida Institute of Engineering & Technology, 19, Knowledge Park-II, Institutional Area, Greater Noida, 201306,India
Keywords:
Cervical cancer, C33A, Rutin, apoptosis, cell cycle, ROS.
Abstract:
Background: Nowadays, the potential therapeutic role of various bioflavonoids including
Curcumin, Luteolin and Resveratrol has currently been well-documented in a vast range of fatal complications
including synaptic failure and cancers. These bioflavonoids are widely being implemented
for the treatment of various cancers as they possess anti-cancerous, anti-oxidant and anti-inflammatory
properties. Moreover, they are also used as a better alternative to conventional therapies since; these
are non-toxic to cells and having no or least side effects. Notably, the pertinent therapeutic role of Rutin
in cervical cancer is still unsettled however, its anti-cancerous role has already been reported in
other cancers including prostate and colon cancer. Rutin (Vitamin P or Rutoside) is a polyphenolics
flavonoid exhibiting multi-beneficial roles against several carcinomas.
Objective: Despite the evidence for its several biological activities, the anticancer effects of Rutin on
human cervical cancer (C33A) cells remain to be explored. In this study, the anticancer potential of
Rutin was investigated by employing the key biomarkers such as nuclear condensation reactive oxygen
species (ROS), apoptosis, and changes in mitochondrial membrane potential (MMP).
Results: Our findings showed that Rutin treatment reduced the cell viability, induced significant increase
in ROS production and nuclear condensation in dose-dependent manner. Moreover, Rutin provoked
apoptosis by inducing decrease in MMP and activation of caspase-3. Cell cycle analysis further
confirmed the efficacy of Rutin by showing cell cycle arrest at G0/G1 phase.
Conclusion: Thus, our study is envisaged to open up interests for elucidating Rutin as an anticancerous
agent against cervical cancer.
