The Open Veterinary Science Journal ISSN: 1874-3188

[DOI: 10.2174/1874318808002010011]
Tumorcidal Effect of Recombinant Newcastle Disease Virus
Katsuro Hagiwara, Tsuyoshi Kadosawa and Takaaki Nakaya Pp 11-15

We have generated GFP expressing recombinant Newcastle Disease Virus (rNDV-GFP) based on a live vaccine strain which contains a less pathogenic cleavage site of F protein leading to restricted viral growth in vivo. We evaluated the rNDV have an effect of oncolytic activity to B16 melanoma cell. The rNDV-GFP infected efficiency to B16 cells and induced cell death. Apoptosis-related genes, Bax and Caspase 3, were elevated in the infected cells. In order to evaluate the tumorcidal effect of rNDV on canine tumor cells, two canine tumors such as highly metastasizing canine osteosarcoma (HMPOS) and transitional cell carcinoma (TCC) were infected with rNDV. The rNDV induced cell death to canine tumor cells within 48 hours post infection. These results suggest that rNDV-GFP have a tumorcidal activity to B16 mouse melanoma and canine tumors in vitro.




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