Solid Lipid Nanoparticles Loaded with Fluorescent-labelled Cyclosporine A: Anti-Inflammatory Activity In Vitro
Marina Gallarate, Loredana Serpe, Federica Foglietta, Gian Paolo Zara, Susanna Giordano, Elena Peira, Daniela Chirio and Luigi BattagliaAffiliation:
Universita degli Studi di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via Pietro Giuria 9, Torino, Italy.
AbstractFMOC-isocyclosporine A, a fluorescent labeled cyclosporine A, was encapsulated in solid lipid nanoparticles (SLN) prepared by the coacervation technique, and its anti-inflammatory activity was evaluated. The anti-inflammatory activity of the fluorescent labelled molecule, measured as inhibition of TNF-α secretion, is similar to the native one. SLN were compared to commercial formulations, through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. Drug-loaded SLN inhibit TNF-α secretion in a lower extent than commercial formulations, probably due to a lower uptake by the cells, but the increase of IL-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity.
Coacervation, cyclosporine A, cytokines, FMOC, inflammation, solid lipid nanoparticles.
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