A Surfactin Lipopeptide Adjuvanted Hepatitis B Vaccines Elicit Enhanced Humoral and Cellular Immune Responses in Mice

ISSN: 1875-5305 (Online)
ISSN: 0929-8665 (Print)

Volume 24, 12 Issues, 2017

Download PDF Flyer

Protein & Peptide Letters

This journal supports open access

Aims & ScopeAbstracted/Indexed in

Submit Abstracts Online Submit Manuscripts Online

Prof. Ben M. Dunn
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
Email: bdunn@ufl.edu

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 1.069
5 - Year: 1.104

A Surfactin Lipopeptide Adjuvanted Hepatitis B Vaccines Elicit Enhanced Humoral and Cellular Immune Responses in Mice

Protein & Peptide Letters, 21(9): 901-910.

Author(s): Hui Pan, Xiuyun Zhao, Zhenqiu Gao and Gaofu Qi.

Affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.


WH1fungin (WF), a lipopeptide surfactin, has been verified as an immunoadjuvant previously. In this study, mice were intranasally or parenterally immunized with WF plus Hepatitis B surface antigen (HBsAg), then the immune responses were detected. The results showed 50 µg WF plus 20 μg HBsAg for intranasal and 10 μg WF plus 1 µg HBsAg for parenteral immunization was efficient inducing strong immune response against HBsAg in mice. A high titer and longterm anti-HBsAg IgG was observed for more than 19 weeks in intranasal or parenteral immunizations, much higher than that induced by CpG or Alum adjuvant. The anti-HBsAg IgA was also induced in intestine and lung, indicating that mucosal as well as systemic immune response was elicited for intranasal immunization. The IgG isotype in serum revealed WF induced a Th2-bias immune response with a higher titer of anti-HBsAg IgG1 than IgG2a in mice. Moreover, WF also induced more Th1 cells producing interferon (IFN)-γ and stronger cytotoxic T lymphocytes response than controls for intramuscular administration. These data further confirmed that WF induced Th1- as well as Th2- type immune response toward HBsAg. Taken together, WF-adjuvanted HBsAg elicits more effective immune response than that adjuvanted by Alum or CpG, suggesting its potential for development of more efficient HBV vaccines in the future.


Adjuvant, HBsAg, immune response, surfactin, vaccine.

Purchase Online Order Reprints Order Eprints Rights and Permissions

Article Details

Volume: 21
Issue Number: 9
First Page: 901
Last Page: 910
Page Count: 10
DOI: 10.2174/0929866521666140418100743
Price: $58

Related Journals

Webmaster Contact: urooj@benthamscience.org Copyright © 2016 Bentham Science