A Screening Method for Potential Anti-Pancreatic Cancer Candidate Compounds Based on Hsp90-Cdc37 Interaction Model in vitro

ISSN: 1875-628X (Online)
ISSN: 1570-1808 (Print)


Volume 11, 10 Issues, 2014


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A Screening Method for Potential Anti-Pancreatic Cancer Candidate Compounds Based on Hsp90-Cdc37 Interaction Model in vitro

Author(s): Hai Zhang, Sen Sun, Qingshan Chen, Guoqing Zhang, Zhenqing Zhang, Duxin Sun and Wenpeng Zhang

Affiliation: Department of Pharmaceutical Science College of Pharmacy University of Michigan Ann Arbor 48109 USA.

Abstract

The Hsp90/Cdc37 (heat shock protein 90; cell division cycle 37) interaction is a promising target for anti-cancer candidate drug development. The purpose of this research is to establish a screening method for anti-pancreatic cancer candidate compounds based on the Hsp90/Cdc37 interaction model in vitro by Split Renilla luciferase protein fragment-assisted complementation bioluminescence technology. The parameters influencing the protein binding in this model were studied, including protein concentration, reaction time, and substrate concentration. The optimized condition was as follows: protein concentration 2μM, the ratio of Hsp90 and Cdc37 1:1, reaction time 10 min, and the GLO® substrate (Promega, Madison, WI, USA) dilution 1:100. The screening model was further validated by some known compounds blocking the Hsp90/Cdc37 interactions. It was proved that this method is rapid and sensitive and could be used for screening of anti-pancreatic cancer candidate compounds.

Keywords: Anti-cancer, Cdc37, A screening method, Hsp90, Hsp90/Cdc37 interaction, Protein interaction model in vitro, SRL-PFAC

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Article Details

Volume: 11
First Page: 1
Last Page: 6
Page Count: 6
DOI: 10.2174/1570180811666140725185235
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