Assessment of a Synthetic Steroid and Flutamide on Dopamine, GSH and H<sub>2</sub>O<sub>2</sub> Levels in Rat Brain in Presence of Fructose

ISSN: 2212-3873 (Online)
ISSN: 1871-5303 (Print)


Volume 14, 4 Issues, 2014


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Endocrine, Metabolic & Immune Disorders - Drug Targets

Formerly: Current Drug Targets - Immune, Endocrine & Metabolic Disorders

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Editor-in-Chief:
Emilio Jirillo
Universitá degli Studi di Bari
Dipartimento di Clinica Medica
Immunologia e Malattie Infettive
Sezione di Microbiologia e Immunologia
Piazza Giulio Cesare-Policlinico
Bari
Italy


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Assessment of a Synthetic Steroid and Flutamide on Dopamine, GSH and H2O2 Levels in Rat Brain in Presence of Fructose

Author(s): David C. Guzman, Eugene Bratoeff, Aylin S. Ortiz, Ernestina H. Garcia, Norma O. Brizuela, Gerardo B. Mejia, Hugo J. Olguin, Daniel S. del Angel and Israel M. Cruz

Affiliation: Laboratorio de Farmacología, Instituto Nacional de Pediatría, Av Iman Nun 1, 3 er piso, Col Cuicuilco, CP 04530, Mexico City, Mexico.

Abstract

Flutamide is a drug used in the treatment of androgen-dependent disorders. However, this treatment is usually accompanied by some adverse side effects. The aim of this work was to analyse the effect of flutamide and to compare this effect with that of a synthetic steroid - 3β-propionyloxy-5-androsten-17-one (PPA) - on levels of dopamine and some oxidative stress markers. For this, thirty-six male young Wistar rats (65g) were recruited and divided into 6 groups. The groups were then treated as follows: Group 1 (control), dimethyl sulfoxide (DMSO); group 2, flutamide (4mg/kg); group 3, PPA; group 4, DMSO + fructose; group 5, flutamide + fructose; and group 6, PPA + fructose. The treatments were administered intraperitoneally at a daily dose of 4 mg/kg for 10 days. In the last day of treatment, blood samples were obtained and used to assess the levels of glucose and cholesterol. The animals were then sacrificed and their prostate gland and brains were obtained for measurement of 5α-reductase, glutathione (GSH), calcium, H2O2, and dopamine in cortex, hemispheres, and medulla/oblongata, using previously validated methods.

Results: Dopamine levels decreased while GSH increased significantly in cortex and hemispheres of animals that received PPA plus fructose. Also in the same group, GSH decreased in cerebellum/medulla oblongata when compared with control group. Peroxidation decreased significantly in all tissues of the groups, while ATPase activity witnessed a significant decrease in cortex and an increase in hemispheres of animal groups treated with flutamide and PPA both in combination with fructose.

Conclusion: The steroid, 3β-propionyloxy-5-androsten-17-one, may in part act as a neuroprotector mediated by the increase of GSH and decrease of H2O2. Besides, imbalance in steroid homeostasis may alter the metabolism of dopamine.


Keywords: Brain, oxidative stress, peroxidation, prostate, rat, synthetic steroid.

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Article Details

Volume: 14
Issue Number: 2
First Page: 126
Last Page: 133
Page Count: 8
DOI: 10.2174/1871530314666140331102306
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