Association of β-tubulin, F-box/Leucine-Rich Repeat Protein 14, and Type 1 Procollagen C-peptide in Bovine Periosteal Cells

ISSN: 2211-5439 (Online)
ISSN: 2211-5420 (Print)


Volume 3, 2 Issues, 2014


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Current Tissue Engineering

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Editor-in-Chief:
Herman S. Cheung
Department of Biomedical Engineering
University of Miami
Miami, FL 33101
USA


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Association of β-tubulin, F-box/Leucine-Rich Repeat Protein 14, and Type 1 Procollagen C-peptide in Bovine Periosteal Cells

Author(s): Mari Akiyama

Affiliation: Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, Japan.

Abstract

The noncollagenous proteins UACA, EXOSC9, and TMX2 were previously identified in bovine periosteal cells with a combination of mass spectrometry and immunohistochemistry. In this study, the proteins β-tubulin, F-box/leucinerich repeat protein 14 (FBXL14), and type 1 procollagen C-peptide were investigated in these cells. Peptides of β-tubulin and FBXL14 were detected with liquid chromatography tandem mass spectrometry, with protein sequence coverages of 2% and 3%, respectively. A two-dimensional protein electrophoretic map of the culture supernatant of bovine periosteal cells suggested that TMX2 was close to type 1 procollagen C-peptide in the cell aggregate. Therefore, an immunohistochemical analysis of β-tubulin, FBXL14, and type 1 procollagen C-peptide was performed. The bovine periosteal cells were positive for β-tubulin, FBXL14, and type 1 procollagen C-peptide. The noncollagenous proteins β-tubulin and FBXL14 were thus identified, like UACA, EXOSC9, and TMX2, in bovine periosteal cells. Type 1 procollagen C-peptide was only expressed in a subset of cells, whereas type 1 collagen was expressed ubiquitously. It is possible that type 1 procollagen C-peptide is associated with those noncollagenous proteins. This study demonstrates the utility of a technique combining MS and immunohistochemistry.

Keywords: β-tubulin, bovine periosteal cells, F-box/leucine-rich repeat protein 14, scaffold-free, type 1 procollagen C-peptide.

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Article Details

Volume: 3
Issue Number: 1
First Page: 2
Last Page: 6
Page Count: 5
DOI: 10.2174/2211542003666140617000141
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