Effect of Internal Structure of Collagen/hydroxyapatite Scaffold on the Osteogenic Differentiation of Mesenchymal Stem Cells

ISSN: 2212-3946 (Online)
ISSN: 1574-888X (Print)


Volume 9, 6 Issues, 2014


Download PDF Flyer




Current Stem Cell Research & Therapy

Aims & ScopeAbstracted/Indexed in


Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Anthony Atala
Wake Forest University School of Medicine,
Medical Center Boulevard
Winston Salem, NC 27157
USA


View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 2.861
5 - Year: 3.00

Effect of Internal Structure of Collagen/hydroxyapatite Scaffold on the Osteogenic Differentiation of Mesenchymal Stem Cells

Author(s): Guobao Chen, Yonggang Lv, Chanjuan Dong and Li Yang

Affiliation: Bioengineering College, Chongqing University 174 Shazheng Jie, Shapingba, Chongqing 400044, China

Abstract

Consisting of seed cells and scaffold, regenerative medicine provides a new way for the repair and regeneration of tissue and organ. Collagen/hydroxyapatite (HA) biocomposite scaffold is highlighted due to its advantageous features of two major components of bone matrix: collagen and HA. The aim of this study is to investigate the effect of internal structure of collagen/HA scaffold on the fate of rat mesenchymal stem cells (MSCs). The internal structure of collagen/HA scaffold was characterized by micro-CT. It is found that the porosity decreased while average compressive modulus increased with the increase of collagen proportion. Within the collagen proportion of 0.35%, 0.5% and 0.7%, the porosities were 89.08%, 78.37% and 75.36%, the pore sizes were 140.6±75.5 μm, 133.9±48.4 μm and 160.7±119.6 μm, and the average compressive moduli were 6.74±1.16 kPa, 8.82±2.12 kPa and 23.61±8.06 kPa, respectively. Among these three kinds of scaffolds, MSCs on the Col 0.35/HA 22 scaffold have the highest viability and the best cell proliferation. On the contrary, the Col 0.7/HA 22 scaffold has the best ability to stimulate MSCs to differentiate into osteoblasts in a relatively short period of time. In vivo research also demonstrated that the internal structure of collagen/HA scaffold has significant effect on the cell infiltration. Therefore, precise control of the internal structure of collagen/HA scaffold can provide a more efficient carrier to the repair of bone defects.

Keywords: Bone tissue engineering, collagen/hydroxyapatite scaffold, differentiation, internal structure, mesenchymal stem cells

Purchase Online Order Reprints Order Eprints Rights and Permissions

  
  



Article Details

Volume: 9
First Page: 1
Last Page: 10
Page Count: 10
DOI: 10.2174/1574888X09666140812112631
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science