Establishment of Colloidal Gold Immunochromatography Strip for Detection of Florfenicol Residues

ISSN: 1875-676X (Online)
ISSN: 1573-4129 (Print)


Volume 10, 4 Issues, 2014


Download PDF Flyer




Current Pharmaceutical Analysis

Aims & ScopeAbstracted/Indexed in


Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK
Email: cpa@benthamscience.org

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 0.771
5 - Year: 1.107

Establishment of Colloidal Gold Immunochromatography Strip for Detection of Florfenicol Residues

Author(s): Airong Zhu, Rulin Huo, Wei Zhou, Lin Zhang, Feng Gao and Guanghong Zhou

Affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P. R. China.

Abstract

Florfenicol is a synthetic and broad-spectrum antibiotic and developed for veterinary use. In this study, onestep membrane-based competitive colloidal gold-based immunochromatographic formats for the rapid detection of florfenicol were developed. The quality of colloidal gold particles was monitored by UV-vis spectroscopy. For the conjugation of colloidal gold and antibody, 24 μg/mL of antibody was confirmed to the optimal amount for stabilization of colloidal gold. With the prepared colloidal gold immunochromatography test strip was determined the standard florfenicol solution. The results demonstrated a detection limit of florfenicol was approximately 1.0 μg/mL. The study showed that the strip had a high sensitivity to florfenicol and without cross-reaction for other antibiotics. The results of 80 animal samples tests confirmed that 0 sample was higher than the detection limits by liquid chromatography-mass spectrometry (LC-MS). There was evidence to suggest that colloidal gold particles and GAB gold-labeled antibody were synthesized successfully. The prepared colloidal gold immunochromatography strip could be used for preliminary screening of florfenicol.




Keywords: Colloidal gold, florfenicol, immunochromatography, LC-MS.

Purchase Online Rights and Permissions

  
  



Article Details

Volume: 10
Issue Number: 4
First Page: 263
Last Page: 270
Page Count: 8
DOI: 10.2174/1573412910666140702162232
Advertisement

Related Journals


Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science