Determination of Amikacin in Different Pharmaceutical Formulations Using a Resonance Rayleigh Scattering Method with Pontamine Sky Blue

ISSN: 1875-676X (Online)
ISSN: 1573-4129 (Print)


Volume 10, 4 Issues, 2014


Download PDF Flyer




Current Pharmaceutical Analysis

Aims & ScopeAbstracted/Indexed in


Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK
Email: cpa@benthamscience.org

View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 0.771
5 - Year: 1.107

Determination of Amikacin in Different Pharmaceutical Formulations Using a Resonance Rayleigh Scattering Method with Pontamine Sky Blue

Author(s): Wenli Han, Fei Qu, Jingchuan Shang, Wei Lu, Junqing Yang and Qing Ma

Affiliation: Department of Pharmacology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, #1 Medical Street, Chongqing 400016, China.

Abstract

Amikacin (AMK) is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong ultraviolet absorbing chromophore or fluorophore. Greatly enhanced resonance Rayleigh scattering (RRS) signals of AMK can be observed with a conventional fluorescence spectrophotometer when AMK interacts with pontamine sky blue (PSB) and forms an ion-association complex in weakly acidic buffer medium. Based on these characteristics, a sensitive assay for detecting AMK was developed. The maximum RRS peak was observed at 362 nm. The enhancement of the RRS signal was directly proportional to the AMK concentration in the range of 0–1.7 µg/ml, and its detection limit (3σ) was 3.0 ng/ml. The method showed a wide linear range and high sensitivity, and almost no interference could be observed from coexistent substances. In addition, this assay was performed in different pharmaceutical formulations of AMK with satisfactory results. Therefore, the proposed method is promising as an effective means for the determination of AMK.

Keywords: Amikacin, resonance Rayleigh scattering, pontamine sky blue, quantitative determination.

Purchase Online Rights and Permissions

  
  



Article Details

Volume: 10
Issue Number: 2
First Page: 105
Last Page: 111
Page Count: 7
DOI: 10.2174/1573412910999140214094944
Advertisement

Related Journals


Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science