Affiliation: USDA-ARS, Soybean Genomics and Improvement Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
To gain new insights into the mechanism of soybean (Glycine max) interaction with the soybean cyst nematode (Heterodera glycines), we compared protein abundance in soybean roots infected by the soybean cyst nematode at different time intervals. Proteins were extracted from roots of 3 and 8 days post-inoculation (dpi) by soybean cyst nematode Heterodera glycines and separated by 2-DE. A difference in abundance was found in a total of 42 protein spots between the susceptible and resistance responses. After in-gel digestion with trypsin, 39 spots representing 33 proteins were subsequently identified by LC-MS/MS. The proteins showing 1.5-fold changes in intensities are related to biochemical processes that may be differentially altered after soybean cyst nematode challenge. The identified proteins belong to the categories of metabolism, energy, cell growth and division, transcription, protein synthesis, protein destination and storage, signal transduction, disease/defense, and secondary metabolism. Taken together, our study provides important information on the use of proteomic methods for studying protein regulation during soybean-soybean cyst nematode interactions.