Transplantation of Undifferentiated Bone-Marrow Stromal Cells into a Vein Graft Accelerates Sciatic Nerve Regeneration in Streptozotocin Induced Diabetic Rats

ISSN: 1875-5739 (Online)
ISSN: 1567-2026 (Print)


Volume 11, 4 Issues, 2014


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Current Neurovascular Research

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Transplantation of Undifferentiated Bone-Marrow Stromal Cells into a Vein Graft Accelerates Sciatic Nerve Regeneration in Streptozotocin Induced Diabetic Rats

Author(s): Rahim Mohammadi, Amin Haghighat and Keyvan Amini

Affiliation: Department of Clinical Sciences Faculty of Veterinary Medicine Urmia University Nazloo Road Urmia,57153 1177 Iran

Abstract

Effect of undifferentiated bone marrow stromal cells (BMSCs) combined with vein graft on peripheral nerve regeneration was studied using a sciatic nerve regeneration model in diabetic rats. A 10-mm sciatic nerve segment was excised and bridged with a vein graft (VG). In VG/BMSC the graft was then filled with 10 microliter undifferentiated BMSCs (2×107 cells /mL). In control group, the graft was only filled with phosphated buffer saline. The regenerated nerve fibers were studied 4, 8 and 12 weeks after surgery. Assessment of nerve regeneration was based on behavioral, functional (Walking Track Analysis), electrophysiological, histomorphometric and immuohistochemical (Schwann cell detection by S100 expression) criteria. The behavioral, functional and electrophysiological studies indicated that there was significant recovery in regeneration of axons in VG/BMSC group (P<0.05). Quantitative morphometric analyses of regenerated fibers showed that the number and diameter of myelinated fibers in VG/BMSC group were significantly higher than in those of the control group (P<0.05). This demonstrates the potential of using undifferentiated BMSCs combined with vein graft in peripheral nerve regeneration in diabetic rats without limitations of donor-site morbidity associated with isolation of Schwann cells. This technique is also cost saving due to reduction in interval from tissue collection until administration of the cells and simplicity of laboratory procedures compared to differentiated BMSCs. It may have clinical implications for the surgical management of diabetic patients after nerve transection


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Article Details

Volume: 11
First Page: 1
Last Page: 1
Page Count: 1
DOI: 10.2174/1567202611666140520124754
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