In Vivo Tracking of Novel SPIO-Molday ION Rhodamine-B™-Labeled Human Bone Marrow-Derived Mesenchymal Stem Cells After Lentivirus- Mediated COX-2 Silencing: A Preliminary Study

ISSN: 1566-5232 (Print)
ISSN: 1875-5631 (Online)


Volume 14, 6 Issues, 2014


Download PDF Flyer




Current Gene Therapy

Aims & ScopeAbstracted/Indexed in

Ranking and Category:
  • 29th of 164 in Genetics & Heredity

Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Ignacio Anegon
Director INSERM UMR 1064-Center for Research in Transplantation and Immunology
CHU de Nantes. 30, boulevard
Nantes
France


View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 4.906
5 - Year: 4.087

In Vivo Tracking of Novel SPIO-Molday ION Rhodamine-B™-Labeled Human Bone Marrow-Derived Mesenchymal Stem Cells After Lentivirus- Mediated COX-2 Silencing: A Preliminary Study

Author(s): Tian He, Yingzhen Wang, Jinyu Xiang and Haining Zhang

Affiliation: Department of Joint Surgery, The Affiliated Hospital of Qingdao University, 59 Haier Rd, 266003, Qingdao, P. R. China.

Abstract

Purpose: Magnetic resonance imaging (MRI) has been used to track magnetically labeled human bone marrowderived mesenchymal stem cells (hBMSCs) in vivo after COX-2 silencing and transplantation into nude rats via tail vein injection. Methods: In the present study, we knocked down COX-2 expression in hBMSCs through lentivirus transduction. The COX-2 knockdown was confirmed by real-time PCR and Western blotting analyses. Subsequently, we labeled cells with the novel reagent SPIO-Molday ION Rhodamine-B™ (MIRB). The viability, proliferation and differentiation of these cells were assessed in vitro. Labeled lenti-shCOX2 hBMSCs, unlabeled hBMSCs and phosphate-buffered saline (PBS) were individually injected into the tail veins of nude rat models, forming three treatment groups. All nude rats underwent GRE T2*-weighted MRI at 1 h, 7 days and 14 days post-injection. After MRI examination, the animals were sacrificed, and the brain and liver were examined by fluorescence microscopy and Prussian Blue staining. Results: Our results confirmed the successful down-regulation of COX-2 at the mRNA and protein levels in hBMSCs by lentivirus transduction. The viability and differentiation of hBMSCs were not affected by MIRB labeling. After 7 days, hypointense signal void areas in the rat livers were observed on MRI. After 14 days, iron particles were detected in the blood vessels, sinusoids, interlobular septum and capsule tissues of the liver. Conclusion: The MIRB-labeled lenti-shCOX2 hBMSCs transplanted into nude rat models via tail vein injection can be detected and monitored in vivo using 3.0 T clinical MRI for up to 14 days after cell transplantation.

Keywords: COX-2, lentivirus magnetic resonance imaging, mesenchymal stem cells, SPIO transplantation.

Purchase Online Rights and Permissions

  
  



Article Details

Volume: 14
Issue Number: 2
First Page: 136
Last Page: 145
Page Count: 10
DOI: 10.2174/1566523214666140408113900
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science