Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic

ISSN: 2212-7186 (Online)
ISSN: 2212-7178 (Print)


Volume 3, 3 Issues, 2016


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Current Environmental Engineering

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Editor-in-Chief:
Jong Moon Park
Department of Chemical Engineering
Pohang University of Science and Technology
Pohang, Kyungbuk
Korea


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Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic



Current Environmental Engineering, 1(1): 4-9.

Author(s): Fei Pan, Ai-Hua Xu, Qing-Fu Zeng, Xin Zhao and Hai-Liang Zhu.

Affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.

Abstract

A yeast strain with high activity of phenol degradation was isolated from activated sludge of a wastewater treatment plant in a coking chemical factory, and identified as Candida tropicalis according to the analysis of VITEK-32 and its 18SrDNA gene sequence. The strain designated as JP-1 was able to grow on phenol as a sole carbon and energy source and it can degrade more than 90% of 1800 mg⋅L-1 phenol within 72h. The optimum pH was 6.0. Kinetic equation of phenol degradation was obtained by regression using MATLAB software. The parameters of Haldane equation were following: µmax=0.387 l⋅hr-1, Ks=4.20 mg⋅L-1, KiKi =465.6 mg⋅L-1. Cell grew was obviously inhibition by high concentration phenol and the inhibition strengthen increased with phenol increasing.

Keywords:

Biodegradation, Candida tropicalis, kinetic, Haldane equation, phenol, wastewater treatment.



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Article Details

Volume: 1
Issue Number: 1
First Page: 4
Last Page: 9
Page Count: 6
DOI: 10.2174/2212717801666140307002510
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