Preparation and in-vitro transfection efficiency evaluation of modified cationic liposome-polyethyleneimine-plasmid nanocomplexes as a novel gene carrier

ISSN: 1875-5704 (Online)
ISSN: 1567-2018 (Print)


Volume 11, 6 Issues, 2014


Download PDF Flyer




Current Drug Delivery

Aims & ScopeAbstracted/Indexed in

Ranking and Category:
  • 126th of 254 in Pharmacology & Pharmacy

Submit Abstracts Online Submit Manuscripts Online

Editor-in-Chief:
Istvan Toth
School of Pharmacy,University of Queensland
Brisbane, 4072
Australia


View Full Editorial Board

Subscribe Purchase Articles Order Reprints

Current: 2.248

Preparation and in-vitro transfection efficiency evaluation of modified cationic liposome-polyethyleneimine-plasmid nanocomplexes as a novel gene carrier

Author(s): Asma Mahmoudi, Reza Kazemi Oskuee, Mohammad Ramezani and Bizhan Malaekeh-Nikoue

Affiliation: School of Pharmacy, Mashhad University of Medical Sciences,Mashhad, Iran

Abstract

Liposome-linear polyethyleneimine (PEI)-DNA nanocomplexes have shown to be an effective non-viral gene delivery vector. In the present study, we tried to improve the transfection efficiency of these nanocomplexes by liposome modification. For this purpose, the lipopolymer prepared by the conjugation of hexylacrylate to the PEI. Liposomes comprising of lipopolymer and DOTAP (1.2-DiOleoyl-3-Trimethyl Ammonium-Propane) were prepared and extruded through polycarbonate filters to obtain desired size. The 2.5, 25 and 250 KDa molecular weights of linear PEI have been used in order to prepare modified liposome-PEI-DNA nanocomplexes. Three C/P ratios of each nanocomplexes were premixed. Size, zeta potential and the DNA condensation ability of these complexes were determined separately, and at the end, the transfection efficiency and cell cytotoxicity of prepared vectors were evaluated on Neuro2A cell line. Mean particle size of all these nanocomplexes was lower than 220 nm with surface charge of 17.5 to 25.9 mV. The lipopolyplexes (comprising of modified liposome:PEI:DNA), modified liposome (as lipoplex) and PEI 250KDa (as polyplex) showed the highest transfection efficacy. This activity was amplified by increasing of carrier to plasmid (C/P) ratio. In addition, the metabolic activity of prepared vectors was 80-100% of control group. In conclusion, the prepared lipopolyplexes showed high ability to enhance gene transfer

Keywords: Liposomes, Lipopolyplexes, Non-viral vector, Polyethyleneimine, Transfection efficiency, Gene delivery

Purchase Online Rights and Permissions

  
  



Article Details

Volume: 11
First Page: 1
Last Page: 7
Page Count: 7
DOI: 10.2174/1567201811666140616160237
Advertisement

Related Journals




Webmaster Contact: urooj@benthamscience.org Copyright © 2014 Bentham Science