Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute

ISSN: 1875-5402 (Online)
ISSN: 1386-2073 (Print)


Volume 17, 10 Issues, 2014


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Combinatorial Chemistry & High Throughput Screening

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  • 23rd of 71 in Chemistry, Applied
  • 47th of 75 in Biochemical Research Methods
  • 152th of 261 in Pharmacology & Pharmacy

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Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute

Author(s): Emma J. Shanks

Affiliation: Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.

Abstract

The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.

Keywords: Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.

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Article Details

Volume: 17
Issue Number: 4
First Page: 328
Last Page: 332
Page Count: 5
DOI: 10.2174/1386207317666140323133249
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