Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute
Emma J. ShanksAffiliation:
Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
AbstractThe RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.
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