Abstract
For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.
Keywords: Hydrophilic interaction liquid chromatography, pharmaceutical application, polar compounds, polar stationary phase, hydrophilic partitioning, hydrogen bonding, electrostatic interactions.
Current Analytical Chemistry
Title:Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations
Volume: 20 Issue: 5
Author(s): Aleksandra Radoičić, Sandra Šegan*, Aleksandra Dramićanin and Dušanka Milojković-Opsenica
Affiliation:
- Institute of Chemistry, Technology, and Metallurgy, University of Belgrade, Njegoševa 12, 11000, Belgrade, Serbia
Keywords: Hydrophilic interaction liquid chromatography, pharmaceutical application, polar compounds, polar stationary phase, hydrophilic partitioning, hydrogen bonding, electrostatic interactions.
Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.
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Cite this article as:
Radoičić Aleksandra, Šegan Sandra*, Dramićanin Aleksandra and Milojković-Opsenica Dušanka, Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations, Current Analytical Chemistry 2024; 20 (5) . https://dx.doi.org/10.2174/0115734110290557240305045032
DOI https://dx.doi.org/10.2174/0115734110290557240305045032 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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